Adenomyosis: single-cell transcriptomic analysis reveals a paracrine mesenchymal-epithelial interaction involving the WNT/SFRP pathway.

ABSTRACT:

Objective

To assess the cellular and molecular landscape of adenomyosis.

Design

Single-cell analysis of genome-wide messenger RNA (mRNA) expression (single-cell RNA sequencing) of matched tissues of endometrium, adenomyosis, and myometrium using relatively large numbers of viable cells.

Setting

Not applicable.

Patient(s)

Patients (n = 3, age range 40-44 years) undergoing hysterectomy for diffuse adenomyosis.

Main outcome measure(s)

Definition of the molecular landscape of matched adenomyotic, endometrial and myometrial tissues from the same uterus using single-cell RNA sequencing and comparison of distinct cell types in these tissues to identify disease-specific cell populations, abnormal gene expression and pathway activation, and mesenchymal-epithelial

SUBMITTER: Yildiz S 

PROVIDER: S-EPMC11257082 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature