Single-fly genome assemblies fill major phylogenomic gaps across the Drosophilidae Tree of Life.

ABSTRACT: Long-read sequencing is driving rapid progress in genome assembly across all major groups of life, including species of the family Drosophilidae, a longtime model system for genetics, genomics, and evolution. We previously developed a cost-effective hybrid Oxford Nanopore (ONT) long-read and Illumina short-read sequencing approach and used it to assemble 101 drosophilid genomes from laboratory cultures, greatly increasing the number of genome assemblies for this taxonomic group. The next major challenge is to address the laboratory culture bias in taxon sampling by sequencing genomes of species that cannot easily be reared in the lab. Here, we build upon our previous methods to perform amplification-free ONT sequencing of single wild flies obtained either directly from the field or from et

SUBMITTER: Kim BY 

PROVIDER: S-EPMC11257246 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature