Thermostabilization of a fungal laccase by entrapment in enzymatically synthesized levan nanoparticles.

ABSTRACT: In this work, we present a comprehensive investigation of the entrapment of laccase, a biotechnologically relevant enzyme, into levan-based nanoparticles (LNPs). The entrapment of laccase was achieved concomitantly with the synthesis of LNP, catalyzed by a truncated version of a levansucrase from Leuconostoc mesenteroides. The study aimed to obtain a biocompatible nanomaterial, able to entrap functional laccase, and characterize its physicochemical, kinetic and thermal stability properties. The experimental findings demonstrated that a colloidal stable solution of spherically shaped LNP, with an average diameter of 68 nm, was obtained. An uniform particle size distribution was observed, according to the polydispersity index determined by DLS. When the LNPs synthesis was performed in the pr

SUBMITTER: Alishah Aratboni H 

PROVIDER: S-EPMC11257323 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature