The cytidine deaminase APOBEC3A regulates nucleolar function to promote cell growth and ribosome biogenesis.

ABSTRACT: Cancer initiates as a consequence of genomic mutations and its subsequent progression relies in part on increased production of ribosomes to maintain high levels of protein synthesis for unchecked cell growth. Recently, cytidine deaminases have been uncovered as sources of mutagenesis in cancer. In an attempt to form a connection between these 2 cancer driving processes, we interrogated the cytidine deaminase family of proteins for potential roles in human ribosome biogenesis. We identified and validated APOBEC3A and APOBEC4 as novel ribosome biogenesis factors through our laboratory's established screening platform for the discovery of regulators of nucleolar function in MCF10A cells. Through siRNA depletion experiments, we highlight APOBEC3A's requirement in making ribosomes and specific

SUBMITTER: McCool MA 

PROVIDER: S-EPMC11257408 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature