Structural Mapping of Polyclonal IgG Responses to HA After Influenza Virus Vaccination or Infection.

ABSTRACT: Cellular and molecular characterization of immune responses elicited by influenza virus infection and seasonal vaccination have informed efforts to improve vaccine efficacy, breadth, and longevity. Here, we use negative stain electron microscopy polyclonal epitope mapping (nsEMPEM) to structurally characterize the humoral IgG antibody responses to hemagglutinin (HA) from human patients vaccinated with a seasonal quadrivalent flu vaccine or infected with influenza A viruses. Our data show that both vaccinated and infected patients had humoral IgGs targeting highly conserved regions on both H1 and H3 subtype HAs, including the stem and anchor, which are targets for universal influenza vaccine design. Responses against H1 predominantly targeted the central stem epitope in infected patients an

SUBMITTER: Leon AN 

PROVIDER: S-EPMC11257458 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature