Long-read RNA sequencing reveals allele-specific N⁶-methyladenosine modifications.

ABSTRACT: Long-read sequencing technology enables highly accurate detection of allele-specific RNA expression, providing insights into the effects of genetic variation on splicing and RNA abundance. Furthermore, the ability to directly sequence RNA promises the detection of RNA modifications in tandem with ascertaining the allelic origin of each molecule. Here, we leverage these advantages to determine allele-biased patterns of N⁶-methyladenosine (m6A) modifications in native mRNA. We utilized human and mouse cells with known genetic variants to assign allelic origin of each mRNA molecule combined with a supervised machine learning model to detect read-level m6A modification ratios. Our analyses revealed the importance of sequences adjacent to the DRACH-motif in determining m6A deposition

SUBMITTER: Park D 

PROVIDER: S-EPMC11257478 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature