The WAVE complex forms linear arrays at negative membrane curvature to instruct lamellipodia formation.

ABSTRACT: Cells generate a wide range of actin-based membrane protrusions for various cell behaviors. These protrusions are organized by different actin nucleation promoting factors. For example, N-WASP controls finger-like filopodia, whereas the WAVE complex controls sheet-like lamellipodia. These different membrane morphologies likely reflect different patterns of nucleator self-organization. N-WASP phase separation has been successfully studied through biochemical reconstitutions, but how the WAVE complex self-organizes to instruct lamellipodia is unknown. Because WAVE complex self-organization has proven refractory to cell-free studies, we leverage in vivo biochemical approaches to investigate WAVE complex organization within its native cellular context. With single molecule tracking and

SUBMITTER: Wu M 

PROVIDER: S-EPMC11257481 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature