PRC1.6 localizes on chromatin with the human silencing hub (HUSH) complex for promoter-specific silencing.

ABSTRACT: An obligate step in the life cycle of HIV-1 and other retroviruses is the establishment of the provirus in target cell chromosomes. Transcriptional regulation of proviruses is complex, and understanding the mechanisms underlying this regulation has ramifications for fundamental biology, human health, and gene therapy implementation. The three core components of the Human Silencing Hub (HUSH) complex, TASOR, MPHOSPH8 (MPP8), and PPHLN1 (Periphilin 1), were identified in forward genetic screens for host genes that repress provirus expression. Subsequent loss-of-function screens revealed accessory proteins that collaborate with the HUSH complex to silence proviruses in particular contexts. To identify proteins associated with a HUSH complex-repressed provirus in human cells, we developed a te

SUBMITTER: Rodriguez TC 

PROVIDER: S-EPMC11257501 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature