Global siRNA Screen Reveals Critical Human Host Factors of SARS-CoV-2 Multicycle Replication.

ABSTRACT: Defining the subset of cellular factors governing SARS-CoV-2 replication can provide critical insights into viral pathogenesis and identify targets for host-directed antiviral therapies. While a number of genetic screens have previously reported SARS-CoV-2 host dependency factors, these approaches relied on utilizing pooled genome-scale CRISPR libraries, which are biased towards the discovery of host proteins impacting early stages of viral replication. To identify host factors involved throughout the SARS-CoV-2 infectious cycle, we conducted an arrayed genome-scale siRNA screen. Resulting data were integrated with published datasets to reveal pathways supported by orthogonal datasets, including transcriptional regulation, epigenetic modifications, and MAPK signalling. The identified provi

SUBMITTER: Yin X 

PROVIDER: S-EPMC11257544 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature