Investigating the role of a Tannerella forsythia HtrA protease in host protein degradation and inflammatory response.

ABSTRACT:

Introduction

Degradation of host proteins by bacterial proteases leads to the subversion of the host response and disruption of oral epithelial integrity, which is considered an essential factor in the progression of periodontitis. High-temperature requirement A (HtrA) protease, which is critical for bacterial survival and environmental adaptation, is found in several oral bacteria, including the periodontal pathogen Tannerella forsythia. This study investigated the proteolytic activity of HtrA from T. forsythia and its ability to modulate the host response.

Methods

HtrA of T. forsythia was identified bioinformatically and produced as a recombinant protein. T. forsythia mutants with depleted and restored HtrA production were constructed. The effec

SUBMITTER: Bloch S 

PROVIDER: S-EPMC11257890 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature