Development of KoRV-pseudotyped lentiviral vectors for efficient gene transfer into freshly isolated immune cells.

ABSTRACT: Allogeneic cell therapies, such as those involving macrophages or Natural Killer (NK) cells, are of increasing interest for cancer immunotherapy. However, the current techniques for genetically modifying these cell types using lenti- or gamma-retroviral vectors present challenges, such as required cell pre-activation and inefficiency in transduction, which hinder the assessment of preclinical efficacy and clinical translation. In our study, we describe a novel lentiviral pseudotype based on the Koala Retrovirus (KoRV) envelope protein, which we identified based on homology to existing pseudotypes used in cell therapy. Unlike other pseudotyped viral vectors, this KoRV-based envelope demonstrates remarkable efficiency in transducing freshly isolated primary human NK cells directly from blood

SUBMITTER: Renner A 

PROVIDER: S-EPMC11257948 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature