Harnessing the dental cells derived from human induced pluripotent stem cells for hard tissue engineering.

ABSTRACT:

Introduction

Most mineralized tissues in our body are present in bones and teeth. Human induced pluripotent stem cells (hiPSCs) are promising candidates for cell therapy to help regenerate bone defects and teeth loss. The extracellular matrix (ECM) is a non-cellular structure secreted by cells. Studies on the dynamic microenvironment of ECM are necessary for stem cell-based therapies.

Objectives

We aim to optimize an effective protocol for hiPSC differentiation into dental cells without utilizing animal-derived factors or cell feeders that can be applied to humans and to mineralize differentiated dental cells into hard tissues.

Methods

For the differentiation of both dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) from hiPSCs, an embryoid body (EB)

SUBMITTER: Kim EJ 

PROVIDER: S-EPMC11258659 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature