A geminivirus crosses the monocot-dicot boundary and acts as a viral vector for gene silencing and genome editing.

ABSTRACT:

Introduction

Members of the family Geminiviridae have been reported to infect either a monocot plant or a dicot plant, but not both. This study reports a geminivirus, Wheat Dwarf India Virus (WDIV), first identified in wheat, that is capable of infecting both monocot and dicot plants and acting as a viral vector.

Objectives

This study was aimed at developing a broad host range viral vector system for reverse genetics and genome editing.

Methods

Here we used a wheat isolate of WDIV and Ageratum yellow leaf curl betasatellite (AYLCB) for infectivity assays and vector development. We performed Agrobacterium-mediated inoculation of WDIV and AYLCB in wheat, oat, barley, corn, soybean, and tobacco. To examine the potential of WDIV to act as a viral vector, we modified the W

SUBMITTER: Kumar J 

PROVIDER: S-EPMC11258672 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature