The cotton MYB33 gene is a hub gene regulating the trade-off between plant growth and defense in Verticillium dahliae infection.

ABSTRACT:

Introduction

Sessile plants engage in trade-offs between growth and defense capacity in response to fluctuating environmental cues. MYB is an important transcription factor that plays many important roles in controlling plant growth and defense. However, the mechanism behind how it keeps a balance between these two physiological processes is still largely unknown.

Objectives

Our work focuses on the dissection of the molecular mechanism by which GhMYB33 regulates plant growth and defense.

Methods

The CRISPR/Cas9 technique was used to generate mutants for deciphering GhMYB33 functions. Yeast two-hybrid, luciferase complementary imaging, and co-immunoprecipitation assays were used to prove that proteins interact with each other. We used the electrophoretic mobility shift

SUBMITTER: Guang H 

PROVIDER: S-EPMC11258673 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature