Biosensors for the detection of chorismate and cis,cis-muconic acid in Corynebacterium glutamicum.

ABSTRACT: Corynebacterium glutamicum ATCC 13032 is a promising microbial chassis for industrial production of valuable compounds, including aromatic amino acids derived from the shikimate pathway. In this work, we developed two whole-cell, transcription factor based fluorescent biosensors to track cis,cis-muconic acid (ccMA) and chorismate in C. glutamicum. Chorismate is a key intermediate in the shikimate pathway from which value-added chemicals can be produced, and a shunt from the shikimate pathway can divert carbon to ccMA, a high value chemical. We transferred a ccMA-inducible transcription factor, CatM, from Acinetobacter baylyi ADP1 into C. glutamicum and screened a promoter library to isolate variants with high sensitivity and dynamic range to ccMA by providing benzoate, which is converted t

SUBMITTER: Velasquez-Guzman JC 

PROVIDER: S-EPMC11258901 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature