Transcriptional dynamics orchestrating the development and integration of neurons born in the adult hippocampus.

ABSTRACT: The adult hippocampus generates new granule cells (aGCs) with functional capabilities that convey unique forms of plasticity to the preexisting circuits. While early differentiation of adult radial glia-like cells (RGLs) has been studied extensively, the molecular mechanisms guiding the maturation of postmitotic neurons remain unknown. Here, we used a precise birthdating strategy to study aGC differentiation using single-nuclei RNA sequencing. Transcriptional profiling revealed a continuous trajectory from RGLs to mature aGCs, with multiple immature stages bearing increasing levels of effector genes supporting growth, excitability, and synaptogenesis. Analysis of differential gene expression, pseudo-time trajectory, and transcription factors (TFs) revealed critical transitions defining fou

SUBMITTER: Rasetto NB 

PROVIDER: S-EPMC11259177 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature