NADPH Alters DUOX1 Calcium Responsiveness.

ABSTRACT: Hydrogen peroxide is a key element in redox signaling and in setting cellular redox tone. DUOX1 and DUOX2, that directly synthesize hydrogen peroxide, are the most abundant NADPH oxidase transcripts in most epithelia. DUOX1 and DUOX2 hydrogen peroxide synthesis is regulated by intracellular calcium transients and thus cells can respond to signals and initiate responses by increasing cellular hydrogen peroxide synthesis. Nevertheless, many details of their enzymatic regulation are still unexplored. DUOX1 and DUOXA1 were expressed in HEK293T cells and activity was studied in homogenates and membrane fractions. When DUOX1 homogenates or membranes were pre-incubated in NADPH and started with addition of Ca²⁺, to mimic intracellular activation, progress curves were distinctly differe

SUBMITTER: Conner GE 

PROVIDER: S-EPMC11259916 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature