Exploring contrast-enhancing staining agents for studying adipose tissue through contrast-enhanced computed tomography.

ABSTRACT: Contrast-enhanced computed tomography offers a nondestructive approach to studying adipose tissue in 3D. Several contrast-enhancing staining agents (CESAs) have been explored, whereof osmium tetroxide (OsO₄) is the most popular nowadays. However, due to the toxicity and volatility of the conventional OsO₄, alternative CESAs with similar staining properties were desired. Hf-WD 1:2 POM and Hexabrix have proven effective for structural analysis of adipocytes using contrast-enhanced computed tomography but fail to provide chemical information. This study introduces isotonic Lugol's iodine (IL) as an alternative CESA for adipose tissue analysis, comparing its staining potential with Hf-WD 1:2 POM and Hexabrix in murine caudal vertebrae and bovine muscle tissue strips. Sing

SUBMITTER: Balcaen T 

PROVIDER: S-EPMC11259937 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature