UPLC-MS based lipidomics analysis on optimization of soybean phosphatidylethanolamine extraction.

ABSTRACT: Soybean-derived phosphatidylethanolamine (PE) is a valuable phospholipid component yet its high-purity form is costly and its molecular structure is poorly understood. The present study combined solvent extraction and cryopurification to purify PE. The optimal extraction conditions were as follows: material-liquid ratio 1:15 (g/mL), ethanol base concentration 100:4 (V_{anhydrous ethanol} /V_{25% ammonia}), extraction temperature 40 °C, time 60 min, extraction twice. The cryopurification conditions were: material-liquid ratio 1:60, ethanol base concentration 100:6 (V_{anhydrous ethanol}/V_{25% ammonia}), freezing temperature - 20 °C, time 20 h. UPLC-QTOF-MS/MS analysis revealed phospholipid composition of raw material, crude product, and purified product. The

SUBMITTER: Wang B 

PROVIDER: S-EPMC11260036 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature