Clustered protocadherin cis-interactions are required for combinatorial cell-cell recognition underlying neuronal self-avoidance.

ABSTRACT: In the developing human brain, only 53 stochastically expressed clustered protocadherin (cPcdh) isoforms enable neurites from individual neurons to recognize and self-avoid while simultaneously maintaining contact with neurites from other neurons. Cell assays have demonstrated that self-recognition occurs only when all cPcdh isoforms perfectly match across the cell boundary, with a single mismatch in the cPcdh expression profile interfering with recognition. It remains unclear, however, how a single mismatched isoform between neighboring cells is sufficient to block erroneous recognitions. Using systematic cell aggregation experiments, we show that abolishing cPcdh interactions on the same membrane (cis) results in a complete loss of specific combinatorial binding between cells (

SUBMITTER: Wiseglass G 

PROVIDER: S-EPMC11260096 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature