Thioredoxin-related transmembrane protein 1 negatively regulates coagulation and phosphatidylserine exposure.

ABSTRACT:

Background

Five secreted platelet protein disulfide isomerases (PDIs) and 1 transmembrane PDI regulate platelet function and thrombosis. Thioredoxin-related transmembrane protein 1 (TMX1) was the first member of the PDI family found to negatively regulate platelet aggregation and platelet accumulation in vivo. The effect of TMX1 on coagulation is unknown.

Objectives

To determine the effect of TMX1 on coagulation.

Methods

TMX1^-/- mice were used to study platelet accumulation and fibrin deposition in vivo in the laser-induced thrombosis injury model. Annexin V deposition at the site of vascular injury was studied using conditional TMX1 knockout mice. Annexin V binding to platelets was studied using human platelets, anti-TMX1 antibodies, and TMX1

SUBMITTER: Zhou J 

PROVIDER: S-EPMC11260325 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature