Expression and characterization of monofunctional alcohol dehydrogenase enzymes in Clostridium thermocellum.

ABSTRACT: Clostridium thermocellum is a thermophilic anaerobic bacterium that could be used for cellulosic biofuel production due to its strong native ability to consume cellulose, however its ethanol production ability needs to be improved to enable commercial application. In our previous strain engineering work, we observed a spontaneous mutation in the native adhE gene that reduced ethanol production. Here we attempted to complement this mutation by heterologous expression of 18 different alcohol dehydrogenase (adh) genes. We were able to express all of them successfully in C. thermocellum. Surprisingly, however, none of them increased ethanol production, and several actually decreased it. Our findings contribute to understanding the correlation between C. th

SUBMITTER: Chiarelli DP 

PROVIDER: S-EPMC11260334 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature