GABA-mediated inhibition of human CD4⁺ T cell functions is enhanced by insulin but impaired by high glucose levels.

ABSTRACT:

Background

γ-aminobutyric acid (GABA), known as the main inhibitory neurotransmitter in the brain, exerts immunomodulatory functions by interaction with immune cells, including T cells. Metabolic programs of T cells are closely linked to their effector functions including proliferation, differentiation, and cytokine production. The physiological molecules glucose and insulin may provide environmental cues and guidance, but whether they coordinate to regulate GABA-mediated T cell immunomodulation is still being examined.

Methods

CD4⁺ T cells that were isolated from blood samples from healthy individuals and from patients with type 1 diabetes (T1D) were activated in vitro. We carried out metabolic assays, multiple proximity extension assay (PEA), ELISA, qPCR, immuno

SUBMITTER: Jin Z 

PROVIDER: S-EPMC11260598 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature