Temporal changes in mouse hippocampus transcriptome after pilocarpine-induced seizures.

ABSTRACT:

Introduction

Status epilepticus (SE) is a seizure lasting more than 5 min that can have lethal consequences or lead to various neurological disorders, including epilepsy. Using a pilocarpine-induced SE model in mice we investigated temporal changes in the hippocampal transcriptome.

Methods

We performed mRNA-seq and microRNA-seq analyses at various times after drug treatment.

Results

At 1 h after the start of seizures, hippocampal cells upregulated transcription of immediate early genes and genes involved in the IGF-1, ERK/MAPK and RNA-PolII/transcription pathways. At 8 h, we observed changes in the expression of genes associated with oxidative stress, overall transcription downregulation, particularly for genes related to mitochondrial structure and function, initiati

SUBMITTER: Popova EY 

PROVIDER: S-EPMC11260795 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature