Simultaneous high throughput genotyping of 36 blood group systems using NGS based on probe capture technology.

ABSTRACT: Human blood group antigen has important biological functions, and transfusion of incompatible blood can cause alloimmunization and may lead to serious hemolytic reactions. Currently, serological methods are most commonly used in blood group typing. However, this technique has certain limitations and cannot fully meet the increasing demand for the identification of blood group antigens. This study describes a next-generation sequencing (NGS) technology platform based on exon and flanking region capture probes to detect full coding exon and flanking intron regions of the 36 blood group systems, providing a new high-throughput method for the identification of blood group antigens. The 871 capture probes were designed for the exon and flanking intron sequences of 36 blood group system genes, a

SUBMITTER: Zhang J 

PROVIDER: S-EPMC11260914 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature