Protocol for matching protein localization to synapse morphology in primary rat neurons by correlative super-resolution microscopy.

ABSTRACT: Super-resolution imaging provides unprecedented visualization of sub-cellular structures, but the two main techniques used, single-molecule localization microscopy (SMLM) and stimulated emission depletion (STED), are not easily reconciled. We present a protocol to super-impose nanoscale protein distribution reconstructed with SMLM to sub-cellular morphology obtained in STED. We describe steps for tracking cells on etched coverslips and registering images from two different microscopes with 30-nm accuracy. In this protocol, synaptic proteins are mapped in the dendritic spines of primary neurons. For complete details on the use and execution of this protocol, please refer to Inavalli et al.¹.

SUBMITTER: Cloatre T 

PROVIDER: S-EPMC11261141 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature