Novel role of MHC class II transactivator in hepatitis B virus replication and viral counteraction.

ABSTRACT:

Background/aims

The major histocompatibility class II (MHC II) transactivator, known as CIITA, is induced by Interferon gamma (IFN-γ) and plays a well-established role in regulating the expression of class II MHC molecules in antigen-presenting cells.

Methods

Primary human hepatocytes (PHH) were isolated via therapeutic hepatectomy from two donors. The hepatocellular carcinoma (HCC) cell lines HepG2 and Huh7 were used for the mechanistic study, and HBV infection was performed in HepG2-NTCP cells. HBV DNA replication intermediates and secreted antigen levels were measured using Southern blotting and ELISA, respectively.

Results

We identified a non-canonical function of CIITA in the inhibition of hepatitis B virus (HBV) replication in both HCC cells and patient-derived

SUBMITTER: Dezhbord M 

PROVIDER: S-EPMC11261224 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature