Multiomics profiling of buffy coat and plasma unveils etiology-specific signatures in hepatocellular carcinoma.

ABSTRACT:

Background/aims

Hepatocellular carcinoma (HCC) is a leading cause of cancer mortality worldwide. Despite identification of several biomarkers for HCC diagnosis, challenges such as low sensitivity and intratumoral heterogeneity have impeded early detection, highlighting the need for etiology-specific blood biomarkers.

Methods

We generated whole-transcriptome sequencing (WTS) and targeted proteome data from buffy coat and plasma samples from HCC patients. By integrating etiological information on viral infection, we investigated the etiology-specific gene expression landscape at the blood level. Validation of differentially expressed genes (DEGs) was performed using publicly available RNA-seq datasets and qRT‒PCR with AUC analyses.

Results

Differential expression analys

SUBMITTER: Hong J 

PROVIDER: S-EPMC11261225 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature