<i>iMAX</i> FRET (Information Maximized FRET) for Multipoint Single-Molecule Structural Analysis.

ABSTRACT: Understanding the structure of biomolecules is vital for deciphering their roles in biological systems. Single-molecule techniques have emerged as alternatives to conventional ensemble structure analysis methods for uncovering new biology in molecular dynamics and interaction studies, yet only limited structural information could be obtained experimentally. Here, we address this challenge by introducing iMAX FRET, a one-pot method that allows ab initio 3D profiling of individual molecules using two-color FRET measurements. Through the stochastic exchange of fluorescent weak binders, iMAX FRET simultaneously assesses multiple distances on a biomolecule within a few minutes, which can then be used to reconstruct the coordinates of up to four points in each molecule, allo

SUBMITTER: Joshi BS 

PROVIDER: S-EPMC11261617 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature