Preparation, identification and molecular docking of two novel anti-aging peptides from perilla seed.

ABSTRACT: Perilla seed meal is an important agricultural by-product of perilla oil extraction. The antioxidant and anti-aging activities of perilla seed meal protein hydrolysate were investigated, and the bioactive peptides were identified to maximize the utilization of perilla seed meal resources. Anti-aging peptides were identified using a combination of peptidomics and in silico bioinformatics. Furthermore, the potential molecular mechanism of these peptides was explored through molecular docking and RT-PCR. The results showed a significant anti-aging properties of F2 (MW 3 kDa ∼5 kDa) by inhibition of reactive oxygen species (ROS) production and β-galactosidase activity. Nine novel peptides were identified from F2 and subsequently synthesized to explore their bioactivities. The two peptides, NFF

SUBMITTER: Wang L 

PROVIDER: S-EPMC11261800 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature