LC3B conjugation machinery promotes autophagy-independent HIV-1 entry in CD4⁺ T lymphocytes.

ABSTRACT: HIV-1 entry into CD4⁺ T lymphocytes relies on the viral and cellular membranes' fusion, leading to viral capsid delivery in the target cell cytoplasm. Atg8/LC3B conjugation to lipids, process named Atg8ylation mainly studied in the context of macroautophagy/autophagy, occurs transiently in the early stages of HIV-1 replication in CD4⁺ T lymphocytes. Despite numerous studies investigating the HIV-1-autophagy interplays, the Atg8ylation impact in these early stages of infection remains unknown. Here we found that HIV-1 exposure leads to the rapid LC3B enrichment toward the target cell plasma membrane, in close proximity with the incoming viral particles. Furthermore, we demonstrated that Atg8ylation is a key event facilitating HIV-1 entry in target CD4⁺ T cel

SUBMITTER: Pradel B 

PROVIDER: S-EPMC11262235 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature