Inhibition of Cathepsin S in Autoimmune CD25KO Mouse Improves Sjogren Disease-Like Lacrimal Gland Pathology.

ABSTRACT:

Purpose

CD25KO mice are a model of Sjögren disease (SjD) driven by autoreactive T cells. Cathepsin S (CTSS) is a protease crucial for major histocompatibility complex class II presentation that primes T cells. We investigated if a diet containing CTSS inhibitor would improve autoimmune signs in CD25KO mice.

Methods

Four-week female CD25KO mice were randomly chosen to receive chow containing a CTSS inhibitor (R05461111, 262.5 mg/kg chow) or standard chow for 4 weeks. Cornea sensitivity was measured. Inflammatory score was assessed in lacrimal gland (LG) histologic sections. Flow cytometry of LG and ocular draining lymph nodes (dLNs) investigated expression of Th1 and Th17 cells. Expression of inflammatory, T- and B-cell, and apoptotic markers in the LG were assessed with quan

SUBMITTER: Scholand KK 

PROVIDER: S-EPMC11262477 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature