Hybridization between Aedes aegypti and Aedes mascarensis mosquitoes leads to disruption of male sex determination.

ABSTRACT: Understanding the sex determination pathway and its disruptions in mosquitoes is critical for the effective control of disease vectors through genetic manipulations based on sex separation. When male hybrids of Aedes aegypti females and Ae. mascarensis males are backcrossed to Ae. aegypti females, a portion of the backcross progeny manifests as males with abnormal sexual differentiation. We discovered a significant correlation between pupal abnormalities and the feminization of subsequent adults exemplified by the relative abundance of ovarian and testicular tissues. All intersex individuals were genetic males as they expressed a male determining factor, Nix. Further, our analysis of the sex-specific splicing of doublesex and fruitless transcripts demonstrated the presence of both male and

SUBMITTER: Liang J 

PROVIDER: S-EPMC11263339 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature