Immunometabolic alteration of CD4⁺ T cells in the pathogenesis of primary Sjogren's syndrome.

ABSTRACT: Primary Sjögren's syndrome (pSS) is a prevalent autoimmune disorder wherein CD4⁺ T cells play a pivotal role in its pathogenesis. However, the underlying mechanisms driving the hyperactivity of CD4⁺ T cells in pSS remain poorly understood. This study aimed to investigate the potential role of immunometabolic alterations in driving the hyperactivity of CD4⁺ T cells in pSS. We employed Seahorse XF assay to evaluate the metabolic phenotype of CD4⁺ T cells, conducted flow cytometry to assess the effector function and differentiation of CD4⁺ T cells and measured the level of intracellular reactive oxygen species (ROS). Additionally, transcriptome sequencing, PCR, and Western blotting were utilized to examine the expression of glycolytic gen

SUBMITTER: Chen Y 

PROVIDER: S-EPMC11263433 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature