METTL3-mediated pre-miR-665/DLX3 m⁶A methylation facilitates the committed differentiation of stem cells from apical papilla.

ABSTRACT: Methyltransferase-like 3 (METTL3) is a crucial element of N6-methyladenosine (m⁶A) modifications and has been extensively studied for its involvement in diverse biological and pathological processes. In this study, we explored how METTL3 affects the differentiation of stem cells from the apical papilla (SCAPs) into odonto/osteoblastic lineages through gain- and loss-of-function experiments. The m⁶A modification levels were assessed using m⁶A dot blot and activity quantification experiments. In addition, we employed Me-RIP microarray experiments to identify specific targets modified by METTL3. Furthermore, we elucidated the molecular mechanism underlying METTL3 function through dual-luciferase reporter gene experiments and rescue experiments. Our findings in

SUBMITTER: Gu T 

PROVIDER: S-EPMC11263550 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature