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DNA-encoded probe-based assay for profiling plant kinase activities.

ABSTRACT: Elucidating kinase-substrate relationships is pivotal for deciphering cellular signaling mechanisms, yet it remains challenging due to the complexity of kinase networks. Herein, we report the development of a versatile DNA-based kinase assay platform for high-throughput profiling of plant protein kinase activities and substrate preferences. Our approach employs DNA-linked peptide substrates, facilitating quantitative and specific kinase activity detection through next-generation DNA sequencing. Leveraging DNA barcodes as quantitative readouts, our approach establishes a high-throughput, sensitive, and specific platform for dissecting kinase-substrate networks in plants, representing a powerful tool for elucidating signaling mechanisms in plants.

SUBMITTER: Chien YC

PROVIDER: S-EPMC11263872 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature

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DNA-encoded probe-based assay for profiling plant kinase activities.

Chien Yuan-Chi YC Valencia C Alexander CA Lee Han Yong HY Yoon Gyeong Mee GM Kim Dongwook D

PNAS nexus 20240719 7

Elucidating kinase-substrate relationships is pivotal for deciphering cellular signaling mechanisms, yet it remains challenging due to the complexity of kinase networks. Herein, we report the development of a versatile DNA-based kinase assay platform for high-throughput profiling of plant protein kinase activities and substrate preferences. Our approach employs DNA-linked peptide substrates, facilitating quantitative and specific kinase activity detection through next-generation DNA sequencing. ...[more]

PMID: 39045014

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