Investigating and Engineering an 1,2-Propanediol-Responsive Transcription Factor-Based Biosensor.

ABSTRACT: Transcription factor (TF)-based biosensors have arisen as powerful tools in the advancement of metabolic engineering. However, with the emergence of numerous bioproduction targets, the variety of applicable TF-based biosensors remains severely limited. In this study, we investigated and engineered an 1,2-propanediol (1,2-PD)-responsive transcription activator, PocR, from Salmonella typhimurium to enrich the current biosensor repertoire. Heterologous characterization of PocR in E. coli revealed a significantly limited operational range and dynamic range, primarily attributed to the leaky binding between PocR and its corresponding promoters in the absence of the 1,2-PD inducer. Promiscuity characterization uncovered the minor responsiveness of PocR toward glycerol and 1,2-butan

SUBMITTER: Teng Y 

PROVIDER: S-EPMC11264322 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature