Meta-analysis Driven Strain Design for Mitigating Oxidative Stresses Important in Biomanufacturing.

ABSTRACT: As the availability of data sets increases, meta-analysis leveraging aggregated and interoperable data types is proving valuable. This study leveraged a meta-analysis workflow to identify mutations that could improve robustness to reactive oxygen species (ROS) stresses using an industrially important melatonin production strain as an example. ROS stresses often occur during cultivation and negatively affect strain performance. Cellular response to ROS is also linked to the SOS response and resistance to pH fluctuations, which is important to strain robustness in large-scale biomanufacturing. This work integrated more than 7000 E. coli adaptive laboratory evolution (ALE) mutations across 59 experiments to statistically associate mutated genes to 2 ROS tolerance ALE conditions from 72

SUBMITTER: Phaneuf PV 

PROVIDER: S-EPMC11264330 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature