Effects of storage conditions on the stability of qPCR reagents: implications for environmental DNA detection.

ABSTRACT:

Objective

Environmental DNA (eDNA) detection is a transformative tool for ecological surveys which in many cases offers greater accuracy and cost-effectiveness for tracking low-density, cryptic species compared to conventional methods. For the use of targeted quantitative PCR (qPCR)-based eDNA detection, protocols typically require freshly prepared reagents for each sample, necessitating systematic evaluation of reagent stability within the functional context of eDNA standard curve preparation and environmental sample evaluation. Herein, we assessed the effects of long-term storage and freeze-thaw cycles on qPCR reagents for eDNA analysis across six assays.

Results

Results demonstrate qPCR plates (containing pre-made PCR mix, primer-probe, and DNA template) remain stable at

SUBMITTER: Lopez MLD 

PROVIDER: S-EPMC11264737 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature