Acinetobacter baumannii satellite phage Aci01-2-Phanie depends on a helper myophage for its multiplication.

ABSTRACT: We report the discovery of a satellite-helper phage system with a novel type of dependence on a tail donor. The Acinetobacter baumannii satellite podovirus Aci01-2-Phanie (short name Phanie) uses a phage phi29-like DNA replication and packaging mode. Its linear 11,885 bp dsDNA genome bears 171 bp inverted terminal repeats (ITR). Phanie is related to phage DU-PP-III from Pectobacterium and to members of the Astrithrvirus from Salmonella enterica. Together, they form a new clade of phages with 27% to 30% identity over the whole genome. Detailed 3D protein structure prediction and mass spectrometry analyses demonstrate that Phanie encodes its capsid structural genes and genes necessary to form a short tail. However, our study reveals that Phanie virions are non-inf

SUBMITTER: Pourcel C 

PROVIDER: S-EPMC11264900 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature