Proteomic investigation of acute and chronic hypoxia/reoxygenation responsive proteins and pathways in H9C2 cardiomyoblasts.

ABSTRACT:

Background/aim

Ischemic heart diseases continue to be a significant global cardiovascular problem in today's world. Myocardial reperfusion (R) is provided with an effective and rapid treatment; however, it can lead to fatal results, as well as ischemia (I). This study aims to use proteomic analysis to assess proteins and pathways in H9C2 cardiomyoblast cells exposed to hypoxic conditions, followed by reoxygenation, representing I/R injury for both short and long terms, reflecting acute and chronic hypoxia, respectively. Utilizing advanced techniques, our goal is to identify and characterize key proteins undergoing alterations during these critical phases.

Materials and methods

H9C2 cardiomyoblasts, a commonly used cell line for simulating in vivo I/R damage, were exposed to

SUBMITTER: Oztug M 

PROVIDER: S-EPMC11265846 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature