Development of multiplex real-time PCR for simultaneous detection of SARS-CoV-2, CCoV, and FIPV.

ABSTRACT:

Introduction

Coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), canine coronavirus (CCoV), and feline infectious peritonitis virus (FIPV), have the potential for interspecies transmission. These viruses can be present in complex environments where humans, dogs, and cats coexist, posing a significant threat to both human and animal safety.

Methods and results

In this study, we developed a novel multiplex TaqMan-probe-based real-time PCR assay for the simultaneous detection and differentiation of SARS-CoV-2, CCoV, and FIPV. Specific primers and TaqMan fluorescent probes were designed based on the N region of SARS-CoV-2 and FIPV, as well as the S region of CCoV, which demonstrated a remarkable sensitivity and specificity toward the targeted v

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11266814 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature