Limb connective tissue is organized in a continuum of promiscuous fibroblast identities during development.

ABSTRACT: Connective tissue (CT), which includes tendon and muscle CT, plays critical roles in development, in particular as positional cue provider. Nonetheless, our understanding of fibroblast developmental programs is hampered because fibroblasts are highly heterogeneous and poorly characterized. Combining single-cell RNA-sequencing-based strategies including trajectory inference and in situ hybridization analyses, we address the diversity of fibroblasts and their developmental trajectories during chicken limb fetal development. We show that fibroblasts switch from a positional information to a lineage diversification program at the fetal period onset. Muscle CT and tendon are composed of several fibroblast populations that emerge asynchronously. Once the final muscle pattern is set, trans

SUBMITTER: Hirsinger E 

PROVIDER: S-EPMC11267076 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature