Heat stress induced piRNA alterations in pachytene spermatocytes and round spermatids.

ABSTRACT:

Background

Spermatogenesis is a temperature-sensitive process, and elevation in temperature hampers this process quickly and significantly. We studied the molecular effects of testicular heating on piRNAs and gene expression in rat testicular germ cells.

Methods

We generated a cryptorchid rat model by displacing the testis from the scrotal sac (34 °C) to the abdominal area (37 °C) and sacrificed animals after 1 day, 3 days, and 5 days. Pachytene spermatocytes and round spermatids were purified using elutriation centrifugation and percoll gradient methods. We performed transcriptome sequencing in pachytene spermatocytes and round spermatids to identify differentially expressed piRNAs and their probable targets, i.e., TE transcripts and mRNAs.

Results

As a result of hea

SUBMITTER: Mehta P 

PROVIDER: S-EPMC11267754 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature