Novel transcriptional regulation of the GAP promoter in Pichia pastoris towards high expression of heterologous proteins.

ABSTRACT:

Background

Pichia pastoris (Komagataella phaffii) is a promising production host, but the usage of methanol limits its application in the medicine and food industries.

Results

To improve the constitutive expression of heterologous proteins in P. pastoris, four new potential transcription regulators (Loc1p, Msn2p, Gsm1p, Hot1p) of the glyceraldehyde triphosphate dehydrogenase promoter (pGAP) were revealed in this study by using cellulase E4 as reporter gene. On this basis, a series of P. pastoris strains with knockout or overexpression of transcription factors were constructed and the deletion of transcription factor binding sites on pGAP was confirmed. The results showed that Loc1p and Msn2p can inhibit the activity of pGAP, while Gsm1p and Hot1p can enhance the activity of

SUBMITTER: Lin X 

PROVIDER: S-EPMC11267847 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature