β-catenin mediates endodermal commitment of human ES cells via distinct transactivation functions.

ABSTRACT:

Background

β-catenin, acting as the core effector of canonical Wnt signaling pathway, plays a pivotal role in controlling lineage commitment and the formation of definitive endoderm (DE) during early embryonic development. Despite extensive studies using various animal and cell models, the β-catenin-centered regulatory mechanisms underlying DE formation remain incompletely understood, partly due to the rapid and complex cell fate transitions during early differentiation.

Results

In this study, we generated new CTNNB1-/- human ES cells (hESCs) using CRISPR-based insertional gene disruption approach and systematically rescued the DE defect in these cells by introducing various truncated or mutant forms of β-catenin. Our analysis showed that a truncated β-catenin lacking both N

SUBMITTER: Ma X 

PROVIDER: S-EPMC11267888 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature