Genes required for survival and proliferation of Mycoplasma bovis in association with host cells.

ABSTRACT: Mycoplasma bovis is an important emerging pathogen of cattle and bison, but our understanding of the genetic basis of its interactions with its host is limited. The aim of this study was to identify genes of M. bovis required for interaction and survival in association with host cells. One hundred transposon-induced mutants of the type strain PG45 were assessed for their capacity to survive and proliferate in Madin-Darby bovine kidney cell cultures. The growth of 19 mutants was completely abrogated, and 47 mutants had a prolonged doubling time compared to the parent strain. All these mutants had a similar growth pattern to the parent strain PG45 in the axenic media. Thirteen genes previously classified as dispensable for the axenic growth of M. bovis were found to be e

SUBMITTER: Premachandre CK 

PROVIDER: S-EPMC11267936 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature