Sub-genomic RNAi-assisted strain evolution of filamentous fungi for enhanced protein production.

ABSTRACT: Genetic engineering at the genomic scale provides a rapid means to evolve microbes for desirable traits. However, in many filamentous fungi, such trials are daunted by low transformation efficiency. Differentially expressed genes under certain conditions may contain important regulatory factors. Accordingly, although manipulating these subsets of genes only can largely reduce the time and labor, engineering at such a sub-genomic level may also be able to improve the microbial performance. Herein, first using the industrially important cellulase-producing filamentous fungus Trichoderma reesei as a model organism, we constructed suppression subtractive hybridization (SSH) libraries enriched with differentially expressed genes under cellulase induction (MM-Avicel) and cellulase repress

SUBMITTER: Sun X 

PROVIDER: S-EPMC11267940 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature