Single-cell transcriptomic analysis reveals the antiangiogenic role of Mgarp in diabetic retinopathy.

ABSTRACT:

Introduction

Diabetic retinopathy (DR) is a common vascular complication of diabetes mellitus and a leading cause of vision loss worldwide. Endothelial cell (EC) heterogeneity has been observed in the pathogenesis of DR. Elucidating the underlying mechanisms governing EC heterogeneity may provide novel insights into EC-specific therapies for DR.

Research design and methods

We used the single-cell data from the Gene Expression Omnibus database to explore EC heterogeneity between diabetic retinas and non-diabetic retinas and identify the potential genes involved in DR. CCK-8 assays, EdU assays, transwell assays, and tube formation assays were conducted to determine the role of the identified gene in angiogenic effects.

Results

Our analysis identified three distinct EC s

SUBMITTER: Ren L 

PROVIDER: S-EPMC11268071 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature