CryoET of β-amyloid and tau within postmortem Alzheimer's disease brain.

ABSTRACT: A defining pathological feature of most neurodegenerative diseases is the assembly of proteins into amyloid that form disease-specific structures¹. In Alzheimer's disease, this is characterized by the deposition of β-amyloid and tau with disease-specific conformations. The in situ structure of amyloid in the human brain is unknown. Here, using cryo-fluorescence microscopy-targeted cryo-sectioning, cryo-focused ion beam-scanning electron microscopy lift-out and cryo-electron tomography, we determined in-tissue architectures of β-amyloid and tau pathology in a postmortem Alzheimer's disease donor brain. β-amyloid plaques contained a mixture of fibrils, some of which were branched, and protofilaments, arranged in parallel arrays and lattice-like structures. Extracellular vesicles a

SUBMITTER: Gilbert MAG 

PROVIDER: S-EPMC11269202 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature